3DO7
X-ray structure of a NF-kB p52/RelB/DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 106 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 77.640, 124.130, 189.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.190 - 3.050 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Number of reflections | 16342 | |
| <I/σ(I)> | 14.4 | 2.9 |
| Completeness [%] | 91.7 | 60 |
| Redundancy | 14 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 6% PEG 4000, 50 mM NaCl, 0.1% BOG, 10 mM DTT, 10 mM NaCitrate and 2 mM Spermidine, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 10 | 1 | 2 | DTT | ||
| 11 | 1 | 2 | NaCitrate | ||
| 12 | 1 | 2 | Spermidine | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | BOG | ||
| 4 | 1 | 1 | DTT | ||
| 5 | 1 | 1 | NaCitrate | ||
| 6 | 1 | 1 | Spermidine | ||
| 7 | 1 | 2 | PEG 4000 | ||
| 8 | 1 | 2 | NaCl | ||
| 9 | 1 | 2 | BOG |
