3DNT
structures of MDT proteins
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.989 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.540, 84.090, 69.250 |
| Unit cell angles | 90.00, 91.56, 90.00 |
Refinement procedure
| Resolution | 42.580 - 1.660 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.21700 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 | 1.760 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.055 | 0.290 |
| Number of reflections | 89050 | |
| <I/σ(I)> | 10.5 | 3.2 |
| Completeness [%] | 96.0 | 0.98 |
| Redundancy | 4 | 425 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | PEG 600, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
