3DN9
Carboxysome Subunit, CcmK1 C-terminal deletion mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-02 |
| Detector | ADSC quantum Q315 |
| Wavelength(s) | 1.0000000000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.204, 49.194, 49.211 |
| Unit cell angles | 90.65, 91.41, 91.43 |
Refinement procedure
| Resolution | 17.610 - 2.280 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bn4 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.093 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.109 | 0.090 | 0.433 |
| Number of reflections | 16303 | ||
| <I/σ(I)> | 9.7 | ||
| Completeness [%] | 79.8 | 94.9 | 42.8 |
| Redundancy | 1.7 | 1.8 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.6M ammonium sulfate, 0.1M MES, 10% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
