3DN9

Carboxysome Subunit, CcmK1 C-terminal deletion mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-02
Detector	ADSC quantum Q315
Wavelength(s)	1.0000000000
Spacegroup name	P 1
Unit cell lengths	49.204, 49.194, 49.211
Unit cell angles	90.65, 91.41, 91.43

Refinement procedure

Resolution	17.610 - 2.280
R-factor	0.224
Rwork	0.222
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bn4
RMSD bond length	0.013
RMSD bond angle	1.093
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.000	90.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.109	0.090	0.433
Number of reflections	16303
<I/σ(I)>	9.7
Completeness [%]	79.8	94.9	42.8
Redundancy	1.7	1.8	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.6M ammonium sulfate, 0.1M MES, 10% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K