3DMW
Crystal structure of human type III collagen G982-G1023 containing C-terminal cystine knot
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000, 0.979, 1.02, 0.97963 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.980, 21.520, 68.970 |
| Unit cell angles | 90.00, 92.58, 90.00 |
Refinement procedure
| Resolution | 34.450 - 2.300 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.27400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.450 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.075 | 0.240 |
| Number of reflections | 3964 | |
| <I/σ(I)> | 7.3 | 2.3 |
| Completeness [%] | 90.0 | 89 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30% PEG 550, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
