3DMN
The crystal structure of the C-terminal domain of a possilbe DNA helicase from Lactobacillus plantarun WCFS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 99.292, 99.292, 72.365 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.800 - 1.660 |
| R-factor | 0.19291 |
| Rwork | 0.191 |
| R-free | 0.22538 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.660 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.850 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.101 | 0.709 |
| Number of reflections | 25526 | |
| <I/σ(I)> | 52.4 | 4.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.8 | 15.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1M HEPES, 30% (w/v) Jeffamine ED-2001@Reagent pH70, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
