3DMK
Crystal structure of Down Syndrome Cell Adhesion Molecule (DSCAM) isoform 1.30.30, N-terminal eight Ig domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97970 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 118.460, 177.610, 434.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 4.190 |
| R-factor | 0.282 |
| Rwork | 0.280 |
| R-free | 0.32700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v5m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.029 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 4.460 |
| High resolution limit [Å] | 4.190 | 4.190 |
| Rmerge | 0.066 | 0.462 |
| Number of reflections | 34106 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 8.7 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 1.28M Ammonium sulfate, 0.2M MES pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
