3DMB

Crystal structure of a putative general stress family protein (xcc2264) from xanthomonas campestris pv. campestris at 2.30 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-16
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97862,0.91837,0.97939
Spacegroup name	P 32 2 1
Unit cell lengths	105.320, 105.320, 68.340
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.665 - 2.300
R-factor	0.213
Rwork	0.210
R-free	0.26400
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.671
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.665		2.380
High resolution limit [Å]	2.300	4.930	2.300
Rmerge	0.048	0.021	0.642
Number of reflections	19745	3827	3545
<I/σ(I)>	17.56	48.1	2.1
Completeness [%]	99.4	99.3	96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	1.2000M K2HPO4, 0.8000M Na2HPO4, 0.1M Acetate pH 4.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K