3DM3

Crystal structure of a domain of a Replication factor A protein, from Methanocaldococcus jannaschii. NorthEast Structural Genomics target MjR118E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-02
Detector	ADSC QUANTUM 210
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	76.001, 76.001, 249.479
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.760 - 2.400
R-factor	0.225
Rwork	0.225
R-free	0.27400
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.102	0.383
Number of reflections	35883
<I/σ(I)>	10	10
Completeness [%]	100.0	100
Redundancy	6.7	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	100 mM Sodium Citrate, 100mM NaCL, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5	293	100 mM Sodium Citrate, 100mM NaCL, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K