3DM1
Crystal structure of the complex of human chromobox homolog 3 (CBX3) with peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-19 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.28268 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.671, 83.671, 110.288 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.100 - 2.400 |
| R-factor | 0.2219 |
| Rwork | 0.220 |
| R-free | 0.26725 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.826 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.088 | 0.836 |
| Number of reflections | 17856 | |
| <I/σ(I)> | 24.63 | 4.38 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.52 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.5 microliter of the protein solution mixed with with 1.5 microliter of the reservoir solution containing 40% PEG 550 MME, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
