3DKT
Crystal structure of Thermotoga maritima encapsulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-05 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0008 |
Spacegroup name | F 41 3 2 |
Unit cell lengths | 669.040, 669.040, 669.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.867 - 3.104 |
R-factor | 0.22 |
Rwork | 0.219 |
R-free | 0.23900 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.330 |
Data scaling software | XSCALE |
Phasing software | AVE/DM |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.867 | 3.300 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.265 | 0.627 |
Number of reflections | 220152 | 37311 |
<I/σ(I)> | 7.43 | 2.1 |
Completeness [%] | 96.5 | 96.9 |
Redundancy | 8.63 | 8.89 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 292 | 0.1M citrate, 21.5% MPD (v/v), 0.25M ammonium acetate, pH5.1, VAPOR DIFFUSION, SITTING DROP, temperature 292K |