3DKA

Crystal structure of a dinb-like protein (yjoa, bsu12410) from bacillus subtilis at 2.30 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-14
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97956,0.97904
Spacegroup name	P 21 21 21
Unit cell lengths	57.690, 63.340, 82.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.566 - 2.300
R-factor	0.219
Rwork	0.217
R-free	0.26900
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.073
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.566	29.570	2.380
High resolution limit [Å]	2.300	4.930	2.300
Rmerge	0.097	0.035	0.571
Number of reflections	13596	2562	2313
<I/σ(I)>	7.3	18.5	1.58
Completeness [%]	95.9	96.5	90.5
Redundancy	3.63

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	0.2000M NH4OAc, 30.0000% PEG-4000, 0.1M Citrate pH 5.6, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K