3DJV
Bovine Seminal Ribonuclease- cytidine 3' phosphate complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-14 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8088 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.355, 58.997, 81.994 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.600 - 1.600 |
R-factor | 0.17809 |
Rwork | 0.177 |
R-free | 0.20363 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1r5d |
RMSD bond length | 0.007 |
RMSD bond angle | 1.342 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.600 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 31086 | |
<I/σ(I)> | 26.2 | 5.4 |
Completeness [%] | 97.9 | 85.1 |
Redundancy | 4.9 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 30% PEG4000, 0.1M sodium acetate, 0.1M Tris/HCl, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |