3DJQ
Bovine Seminal Ribonuclease- Uridine 5' diphosphate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8040 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.080, 59.206, 82.723 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.200 - 1.530 |
| R-factor | 0.20273 |
| Rwork | 0.201 |
| R-free | 0.23272 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1r5d |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.125 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.200 | 1.560 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Number of reflections | 35662 | |
| <I/σ(I)> | 21.4 | 4.4 |
| Completeness [%] | 97.5 | 76.1 |
| Redundancy | 6.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 30% PEG4000, 0.1M sodium acetate, 0.1 M Tris/HCl, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
