3DJD
Crystal structure of the deglycating enzyme fructosamine oxidase from Aspergillus fumigatus (Amadoriase II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.310, 54.266, 106.252 |
| Unit cell angles | 90.00, 95.92, 90.00 |
Refinement procedure
| Resolution | 36.960 - 1.750 |
| R-factor | 0.16921 |
| Rwork | 0.167 |
| R-free | 0.20445 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.367 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.960 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.087 | 0.623 |
| Number of reflections | 85107 | |
| <I/σ(I)> | 37.4 | 3.9 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 13.3 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.1 M Hepes pH 7.4, 10 % iso-propanol, 18 % PEG 4000, protein solution 15 mg/ml, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
