3DJ3
Crystal Structure of C-terminal Truncated TIP-1 (6-113)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97000 |
| Spacegroup name | P 43 |
| Unit cell lengths | 86.808, 86.808, 81.909 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.030 - 2.400 |
| R-factor | 0.265 |
| Rwork | 0.265 |
| R-free | 0.31400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3diw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.045 | 0.149 |
| Number of reflections | 23790 | |
| <I/σ(I)> | 38 | 10 |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 7.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.18M DL-malic acid, 25% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
