3DIV

Crystal structure of laccase from Cerrena maxima at 1.76A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-12
Detector	MAR CCD 165 mm
Wavelength(s)	0.8068
Spacegroup name	P 21 21 21
Unit cell lengths	52.581, 77.101, 130.884
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.990 - 1.760
R-factor	0.18067
Rwork	0.176
R-free	0.21705
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h5u
RMSD bond length	0.011
RMSD bond angle	1.399
Data reduction software	DENZO
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.070	1.800
High resolution limit [Å]	1.760	1.760
Rmerge	0.180
Number of reflections	52442
Completeness [%]	96.0	91.8
Redundancy	4.87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	50mM sodium citrate, 15%(w/v) PEG 6000, 0.04M Tris, malein, NaOH , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K