3DI0
Crystal Structure of Dihydrodipicolinate synthase from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 118.686, 118.686, 179.156 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.430 - 2.380 |
| R-factor | 0.20509 |
| Rwork | 0.203 |
| R-free | 0.24955 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o5k |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.647 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.500 | 2.480 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.074 | 0.397 |
| Number of reflections | 30624 | |
| <I/σ(I)> | 16 | 3.6 |
| Completeness [%] | 80.8 | |
| Redundancy | 5.5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 2M Ammonium sulfate, 0.1M Bis-Tris pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
