3DHZ
Apo (iron free) structure of C. ammoniagenes R2 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.908, 86.537, 70.610 |
| Unit cell angles | 90.00, 105.48, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.630 |
| R-factor | 0.168 |
| Rwork | 0.165 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.760 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.204 | |
| Number of reflections | 67077 | |
| Completeness [%] | 94.7 | 86.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 30% w/v PEG 4000, 100 mM sodium citrate, 200 mM Ammonium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
