3DHU
Crystal structure of an alpha-amylase from Lactobacillus plantarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 202.279, 63.773, 155.677 |
| Unit cell angles | 90.00, 102.93, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.25100 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.419 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.412 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.183 | 0.566 |
| Total number of observations | 125584 | |
| Number of reflections | 130025 | |
| <I/σ(I)> | 11 | 3.3 |
| Completeness [%] | 99.2 | 97.9 |
| Redundancy | 7.1 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 280mM ammonium sulfate, 30% PEG 4K, pH 7.0, Vapor diffusion, temperature 294K |
