3DHT
The Crystal Structure Determination of Rat (rattus norvegicus) Hemoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 273 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54780 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.710, 53.710, 350.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.260 - 2.980 |
| R-factor | 0.24257 |
| Rwork | 0.239 |
| R-free | 0.27466 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fhj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.125 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.080 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Number of reflections | 6233 | |
| <I/σ(I)> | 3 | |
| Redundancy | 14.3 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 40% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
