3DHQ
Crystal structure of Staphylococcal nuclease variant Delta+PHS A90R at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-03 |
| Detector | BRUKER APEX II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.931, 60.603, 38.421 |
| Unit cell angles | 90.00, 93.93, 90.00 |
Refinement procedure
| Resolution | 30.300 - 2.150 |
| R-factor | 0.215 |
| Rwork | 0.208 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.925 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.300 | 2.250 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.020 | 0.137 |
| Number of reflections | 7790 | |
| <I/σ(I)> | 36.48 | 7.06 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.57 | 9.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 24% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTP, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
