3DH8
Structure of Pseudomonas Quinolone Signal Response Protein PqsE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97881 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.980, 60.980, 146.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.960 - 1.800 |
| R-factor | 0.15809 |
| Rwork | 0.156 |
| R-free | 0.19627 |
| Structure solution method | Rigid body refinement of native structure 2q0i in REFMAC5 |
| Starting model (for MR) | 2q0i |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.207 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.053 | 0.377 |
| Number of reflections | 30110 | |
| <I/σ(I)> | 26 | 4.3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 11 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 292 | 100mM HEPES, 200mM MgCl2, 30% PEG 400, 5mM bis-pNPP, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
