3DGY
Crystal structure of ribonuclease Sa2 with guanosine-2'-cyclophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2003-06-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 101.489, 67.313, 57.234 |
Unit cell angles | 90.00, 100.63, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.21707 |
Rwork | 0.215 |
R-free | 0.24635 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1py3 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.388 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.820 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.054 | 0.317 |
Number of reflections | 34762 | |
<I/σ(I)> | 19.3 | 2.7 |
Completeness [%] | 98.9 | 96.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | ammonium sulfate, phosphate buffer pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |