3DGT
The 1.5 A crystal structure of endo-1,3-beta-glucanase from Streptomyces sioyaensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.509, 75.967, 79.663 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.750 - 1.500 |
| R-factor | 0.18361 |
| Rwork | 0.183 |
| R-free | 0.19832 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.244 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.570 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.048 | 0.098 |
| Number of reflections | 38840 | |
| <I/σ(I)> | 34.8 | 14.4 |
| Completeness [%] | 99.0 | 96.3 |
| Redundancy | 4.2 | 3.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microseeding method | 7.5 | 278 | 25% PEG MME 2000, 100mM Hepes pH 7.5, 10mM MgCl2, 0.01% NaN3, microseeding method, temperature 278K |
