3DGJ
NNFGAIL segment from Islet Amyloid Polypeptide (IAPP or amylin)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.190, 4.897, 31.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.110 - 1.800 |
| R-factor | 0.166 |
| Rwork | 0.160 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.110 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.158 | 0.294 |
| Number of reflections | 481 | 62 |
| <I/σ(I)> | 8.98 | 3.8 |
| Completeness [%] | 98.8 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% ethylene glycol, no buffer, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
