3DFE

Crystal structure of a Putative Pii-Like Signaling Protein (YP_323533.1) from ANABAENA VARIABILIS ATCC 29413 at 2.35 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97854
Spacegroup name	P 1 21 1
Unit cell lengths	63.491, 78.749, 65.544
Unit cell angles	90.00, 100.56, 90.00

Refinement procedure

Resolution	29.814 - 2.350
R-factor	0.227
Rwork	0.224
R-free	0.26700
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.621
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.814	29.820	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.059	0.038	0.606
Total number of observations		1000	7380
Number of reflections	26560
<I/σ(I)>	7.9	15.2	1.3
Completeness [%]	99.9	92.4	100
Redundancy	3.8	3.4	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	35.0000% 2-propanol, 5.0000% PEG-1000, 35.0000% 2-propanol, 5.0000% PEG-1000, 0.1M Citrate pH 5.5, NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 293K