3DFA
Crystal structure of kinase domain of calcium-dependent protein kinase cgd3_920 from Cryptosporidium parvum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.91996 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.447, 69.447, 137.414 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.090 - 2.450 |
| R-factor | 0.234 |
| Rwork | 0.233 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qg5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.014 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.072 | 0.471 |
| Number of reflections | 12641 | |
| <I/σ(I)> | 11.2 | 4.53 |
| Completeness [%] | 96.9 | 83.5 |
| Redundancy | 13.2 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 0.1 M Tris-HCl pH 7.6, 20% PEG 8000, 0.38 M Ammonium sulfate, 25% Ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
