3DF8
The crystal structure of a possible HxlR family transcriptional factor from Thermoplasma volcanium GSS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.739, 54.755, 40.687 |
| Unit cell angles | 90.00, 125.31, 90.00 |
Refinement procedure
| Resolution | 38.300 - 1.650 |
| R-factor | 0.17285 |
| Rwork | 0.171 |
| R-free | 0.21064 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.558 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.300 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.099 | 0.477 |
| Number of reflections | 13735 | |
| <I/σ(I)> | 27.9 | 1.8 |
| Completeness [%] | 97.3 | 77.3 |
| Redundancy | 4.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 0.2M Cacium Acetate, 0.1M MES 20%(w/v) PEG8000, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
