3DF7
Crystal structure of a putative ATP-grasp superfamily protein from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2008-06-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.492, 60.450, 119.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.200 - 1.870 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.22600 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.060 | 0.217 |
| Number of reflections | 25633 | |
| <I/σ(I)> | 12.3 | 5 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 13.9 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M sodium cacodylate (pH 6.5), 30% PEG8000, 0.2M sodium acetate , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
