3DEW
The structure of a putative TetR family transcriptional regulator from Geobacter sulfurreducens PCA.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915,0.97937 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.106, 63.106, 76.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.500 - 1.750 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.21000 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.430 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.500 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.074 | 0.483 |
| Number of reflections | 18099 | |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 10.8 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.1M Na Acetate pH4.6, 4% PEG 4K, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
