3DEL
The structure of CT381, the arginine binding protein from the periplasm Chlamydia trachomatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97995 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.072, 121.875, 170.097 |
| Unit cell angles | 90.00, 95.38, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.920 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.21330 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.127 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 2.020 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.086 | 0.480 |
| Number of reflections | 123164 | |
| <I/σ(I)> | 13.8 | 2.1 |
| Completeness [%] | 99.5 | 96.6 |
| Redundancy | 4.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | Sodium Formate 0.5M-4M, Tris Base 100mM, pH 6.8-8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
