3DEF
Crystal structure of Toc33 from Arabidopsis thaliana, dimerization deficient mutant R130A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.440, 71.440, 112.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.780 - 1.960 |
| R-factor | 0.17311 |
| Rwork | 0.171 |
| R-free | 0.21346 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bb3 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.757 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0008) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.990 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Number of reflections | 21395 | |
| <I/σ(I)> | 37.2 | 4.4 |
| Completeness [%] | 97.3 | 96.5 |
| Redundancy | 6.9 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 292 | 20% PEG 3350, 0.2M NH4Cl, 20% Glycerol, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
