3DED
C-terminal domain of Probable hemolysin from Chromobacterium violaceum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-26 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.144, 55.312, 93.734 |
| Unit cell angles | 90.00, 94.34, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.140 |
| R-factor | 0.18823 |
| Rwork | 0.186 |
| R-free | 0.23470 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.417 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.140 | 2.150 |
| Rmerge | 0.125 | 0.386 |
| Number of reflections | 37093 | |
| <I/σ(I)> | 21.34 | 3.84 |
| Completeness [%] | 98.8 | 98.2 |
| Redundancy | 6.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.1M NaCacodylate pH 6.5, 0.2M Ca(OAC), 9%PEG4K., VAPOR DIFFUSION, HANGING DROP, temperature 289K |
