3DDV
The crystal structure of the transcriptional regulator (GntR family) from Enterococcus faecalis V583
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 86.153, 86.153, 240.059 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 80.060 - 2.650 |
| R-factor | 0.20828 |
| Rwork | 0.206 |
| R-free | 0.25115 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.008 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.060 | 2.718 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.124 | 0.643 |
| Number of reflections | 28847 | |
| <I/σ(I)> | 47.9 | 1.88 |
| Completeness [%] | 98.1 | 75.68 |
| Redundancy | 13.4 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.1M Tris, 2M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
