3DDT
Crystal structure of the B2 box from MuRF1 in dimeric state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.992 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 76.220, 76.220, 146.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.694 - 1.900 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.25190 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.358 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 20520 | |
| <I/σ(I)> | 20.7 | 7.8 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 10.4 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 1.8M ammonium sulfate, 0.1M Tris pH 8.5, 15% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 1.8M ammonium sulfate, 0.1M Tris pH 8.5, 15% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
