3DD6
Crystal structure of Rph, an exoribonuclease from Bacillus anthracis at 1.7 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.931 |
Spacegroup name | H 3 2 |
Unit cell lengths | 86.750, 86.750, 179.080 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.650 - 1.702 |
R-factor | 0.21384 |
Rwork | 0.212 |
R-free | 0.24414 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oyr |
RMSD bond length | 0.012 |
RMSD bond angle | 1.462 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.360 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 28380 | |
<I/σ(I)> | 25.2 | 4.8 |
Completeness [%] | 98.7 | 95.6 |
Redundancy | 7.3 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | Ammonium sulphate, K/Na tartrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |