3DCN
Glomerella cingulata apo cutinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.000, 60.000, 86.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.250 - 1.900 |
| R-factor | 0.196 |
| Rwork | 0.190 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cex |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.513 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.340 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.062 | 0.339 |
| Number of reflections | 13027 | |
| <I/σ(I)> | 10.7 | 5.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.2 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 290 | 28% PEG 4000, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
