3DCM
Crystal structure of the Thermotoga maritima SPOUT family RNA-methyltransferase protein Tm1570 in complex with S-adenosyl-L-methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-21 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.648, 63.402, 54.522 |
| Unit cell angles | 90.00, 129.03, 90.00 |
Refinement procedure
| Resolution | 17.430 - 2.000 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.23500 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.417 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.071 | 0.247 |
| Number of reflections | 14038 | |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 98.4 | 91.2 |
| Redundancy | 4.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 100mM Tris-HCl pH 7.0, 200mM calcium acetate, 20% (v/v) PEG 3000, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 100mM Tris-HCl pH 7.0, 200mM calcium acetate, 20% (v/v) PEG 3000, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
