3DCD

X-ray structure of the galactose mutarotase related enzyme Q5FKD7 from Lactobacillus acidophilus at the resolution 1.9A. Northeast Structural Genomics consortium target LaR33.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-22
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	53.270, 88.270, 139.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.740 - 1.900
R-factor	0.17936
Rwork	0.178
R-free	0.20607
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.302
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.072	0.219
Number of reflections	99285
<I/σ(I)>	30.2	9.2
Completeness [%]	99.5	95.2
Redundancy	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.1M KNO3, 0.1M Tris HCl, 24% PEG8K, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K