3DCA
Crystal structure of the RPA0582- protein of unknown function from Rhodopseudomonas palustris- a structural genomics target
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | H 3 |
| Unit cell lengths | 193.495, 193.495, 118.848 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.590 - 3.350 |
| R-factor | 0.274 |
| Rwork | 0.273 |
| R-free | 0.29000 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.685 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0017) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.590 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.088 | 0.426 |
| Number of reflections | 22256 | |
| <I/σ(I)> | 13.716 | 1.5 |
| Completeness [%] | 93.6 | 93.2 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M AMMONIUM SULPHATE, 0.1M NA CACODILATE PH6.5, 30%PEG8K, , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
