3DBV
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE MUTANT WITH LEU 33 REPLACED BY THR, THR 34 REPLACED BY GLY, ASP 36 REPLACED BY GLY, LEU 187 REPLACED BY ALA, AND PRO 188 REPLACED BY SER COMPLEXED WITH NAD+
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 297 |
Detector technology | IMAGE PLATE |
Collection date | 1994-05 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.870, 124.800, 82.920 |
Unit cell angles | 90.00, 108.72, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.450 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.27500 |
RMSD bond length | 0.012 |
RMSD bond angle | 26.100 * |
Data reduction software | XDS |
Data scaling software | Agrovata |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.450 |
Rmerge | 0.078 * |
Number of reflections | 56855 |
Completeness [%] | 96.0 |
Redundancy | 3.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.9 | pH 6.9 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 2 (M) | |
3 | 1 | drop | NAD(P)+ | 30 (mM) | |
4 | 1 | drop | phosphate | 50 (mM) | |
5 | 1 | drop | EDTA | 1 (mM) | |
6 | 1 | drop | dithiothreitol | 1 (mM) | |
7 | 1 | reservoir | ammonium sulfate | 2.5 (M) |