3DB7
Crystal structure of a putative calcium-regulated periplasmic protein (bt0923) from bacteroides thetaiotaomicron at 1.40 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-04 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97947,0.97891 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.170, 48.420, 44.610 |
| Unit cell angles | 90.00, 113.27, 90.00 |
Refinement procedure
| Resolution | 24.922 - 1.400 |
| R-factor | 0.16228 |
| Rwork | 0.160 |
| R-free | 0.19824 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.559 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 24.922 | 24.920 | 1.450 |
| High resolution limit [Å] | 1.400 | 3.010 | 1.400 |
| Rmerge | 0.042 | 0.017 | 0.536 |
| Number of reflections | 23150 | ||
| <I/σ(I)> | 10.44 | 32.1 | 1.5 |
| Completeness [%] | 90.5 | ||
| Redundancy | 2.149 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | 0.2000M CaAcetate, 20.0000% PEG-3350, No Buffer pH 7., NANODROP, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
