3DAE
Crystal structure of phosphorylated SNF1 kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2007-06-24 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.00001 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.607, 72.370, 112.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.192 - 2.899 |
| R-factor | 0.229 |
| Rwork | 0.227 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2EUE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.419 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.200 | 3.000 |
| High resolution limit [Å] | 2.899 | 2.899 |
| Rmerge | 0.087 | 0.382 |
| Total number of observations | 102012 | |
| Number of reflections | 14122 | |
| <I/σ(I)> | 20.7 | 4.29 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | Tris pH 8.5, 3.2-3.6M NH4Ac, vapor diffusion, hanging drop, temperature 298K |
