3D9W
Crystal Structure Analysis of Nocardia farcinica Arylamine N-acetyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2007-03-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.340, 140.920, 74.850 |
| Unit cell angles | 90.00, 101.83, 90.00 |
Refinement procedure
| Resolution | 45.700 - 2.700 |
| Rwork | 0.190 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bsz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.155 | 0.527 |
| Number of reflections | 33425 | |
| <I/σ(I)> | 12.63 | 3.74 |
| Completeness [%] | 97.7 | 94.7 |
| Redundancy | 5.3 | 5.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 15% PEG, 0.8M NaCl, 0.1M Bicine, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
