3D8M

Crystal structure of a chimeric receptor binding protein from lactococcal phages subspecies TP901-1 and p2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-28
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9340
Spacegroup name	P 21 3
Unit cell lengths	85.833, 85.833, 85.833
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.690 - 3.350
R-factor	0.22853
Rwork	0.221
R-free	0.27437
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bsd 2f0c
RMSD bond length	0.013
RMSD bond angle	1.307
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	BALBES
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.750	3.530
High resolution limit [Å]	3.350	3.350
Rmerge	0.245	0.509
Number of reflections	3206
<I/σ(I)>	15.9	6.6
Completeness [%]	100.0	100
Redundancy	19.9	20.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1M Sodium Cacodylate, 12% MPEG2000, pH5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K