3D8A
Co-crystal structure of TraM-TraD complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.11588 |
| Spacegroup name | H 3 |
| Unit cell lengths | 142.246, 142.246, 70.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 2.550 |
| R-factor | 0.21973 |
| Rwork | 0.218 |
| R-free | 0.25746 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g07 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.058 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.070 | 2.560 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 17494 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% PEG 2000, 100 mM tris HCl pH 8.5, 200 mM sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
