3D73
Crystal structure of a pheromone binding protein mutant D35A, from Apis mellifera, at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-14 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.873, 60.859, 56.682 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.030 |
| R-factor | 0.17535 |
| Rwork | 0.172 |
| R-free | 0.22409 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h8v |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.375 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.430 | 2.120 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.057 | 0.254 |
| Number of reflections | 14418 | |
| <I/σ(I)> | 22.2 | 6.4 |
| Completeness [%] | 97.4 | 82.2 |
| Redundancy | 6.2 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.1M ammonium sulfate, 0.2M di-ammonium phosphate, 20mM di-sodium phosphate, pH7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
