3D72
1.65 Angstrom crystal structure of the Cys71Val variant in the fungal photoreceptor VVD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2007-10-27 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.240, 80.700, 57.580 |
| Unit cell angles | 90.00, 95.16, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.650 |
| R-factor | 0.26 |
| Rwork | 0.250 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pd7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.063 | 0.180 |
| Number of reflections | 39115 | |
| <I/σ(I)> | 37.95 | 6.9 |
| Completeness [%] | 98.6 | 94.1 |
| Redundancy | 4.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 26% PEG 4000, 100 mM triSodium Citrate pH 5.6, 100 mM Sodium Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
