3D6R
Structure of an avian influenza A virus NS1 protein effector domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-01 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 65.249, 65.249, 338.767 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.970 - 1.997 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.069 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 26147 | |
| Completeness [%] | 85.9 | 49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 8K, hepes, Na thiocyanate, glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
