3D6Q
The RNase A- 5'-Deoxy-5'-N-piperidinouridine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.094, 32.498, 72.496 |
| Unit cell angles | 90.00, 90.71, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.18016 |
| Rwork | 0.179 |
| R-free | 0.20937 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2g8q |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.443 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 30942 | |
| <I/σ(I)> | 25.8 | 6.7 |
| Completeness [%] | 99.0 | 98.2 |
| Redundancy | 8.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | PEG 4000, SODIUM CITRATE, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
